True Universal Detection

Evaporative light scattering is an ideal detection technique for LC-separated analytes that do not have light-absorbing chromophores. And because this technique does not rely on optical properties, tedious sample preparation and derivatization can be eliminated. The new Agilent 1290 Infinity ELSD takes this capability further by facilitating sub-ambient operation for unrivalled detection of semi-volatile compounds. The three key stages of nebulization, eluent evaporation and optical detection, are optimized by design to achieve maximum sensitivity and highest reproducibility for accurate and reliable results. The nebulizer uses a stream of inert gas to transform the eluent from the LC column into a fine vapor of uniformly sized droplets. Control of nebulizer temperature and gas flow provide for excellent stability and reproducibility. A study of 50 replicate caffeine injections showed peak height variations of less than 2.0 % - thanks to the design of the high-precision glass nebulizer. The evaporator tube temperature is precisely controlled and removes the solvent from the less-volatile analyte particles. The volume of the tube is extremely low, minimizing peak dispersion. In the optical chamber, a short-wavelength, high-power blue laser maximizes the scattering from the small analyte particles. Advanced detection electronics and digital signal processing minimize baseline noise to ensure highest sensitivity. The 1290 Infinity ELSD deploys patented technology to remove the solvent at temperatures as low as 10 degrees centigrade, ensuring compounds are not evaporated prior to detection. This provides for sensitive, reliable quantification of semi-volatile compounds that simply cannot be detected with other evaporative light scattering or aerosol-based detectors. The 1290 Infinity ELSD is unique in its ability to run gas flow gradients, which adjust the size of the nebulized droplets, compensating for mobile phase changes and thereby achieving uniform detector response. The detector operates over the widest flow range and with the steepest gradients without the need to split the flow or to exchange nebulizers. The excellent baseline stability and low dispersion characteristics make it ideal for UHPLC applications. By careful control of the gas flow during evaporation, solvents such as dimethyl sulfoxide can be removed completely, facilitating accurate compound screening without the need for extensive sample preparation. Evaporative light scattering is also an ideal detection technique for supercritical fluid chromatography. With thermally insulated bulkhead fittings and a heated nebulizer, the 1290 Infinity ELSD can easily be coupled to an SFC system without modification. And because SFC provides faster, higher resolution separations than conventional HPLC, laboratory throughput can be increased significantly. The Agilent 1290 Infinity ELSD offers a wealth of productivity benefits such as low temperature evaporation, excellent reproducibility, uniform detector response, widest flow range, lowest dispersion, DMSO transparency and SFC compatibility. The Agilent 1290 Infinity ELSD is the detector of choice for: Analytical and 2-dimensional LC, LC/MS, SFC, Preparative LC, and GPC/SEC The Agilent 1290 Infinity ELSD DETECTS VIRTUALLY EVERYTHING YOUR LC SEPARATES. True universal detection is just one of a wave of innovative technologies to ensure your Agilent 1200 Infinity Series LC stays at the forefront of separation potential.