Discovering the sex of your puppy is fairly straightforward when you know a few anatomical landmarks. Make sure to handle your puppy gently and carefully. If you can, try to wait until the puppy is 3-4 weeks old before trying to determine its sex. If you handle the puppy too much before it has had time to bond with its mother, the mother may reject the puppy. All used images licensed under Creative Commons. Sources: ****** ****** ****** ****** ****** ****** ******